Regulation of β-catenin phosphorylation and nuclear/cytoplasmic transport by APC and its cancer-related truncated form

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Regulation of the phosphorylation and nuclear import and export of β-catenin by APC and its cancer-related truncated form.

We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration that APC is necessary for β-catenin phosphorylation, both for priming of the prot...

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Regulation of the phosphorylation and nuclear import and export of b-catenin by APC and its cancer-related truncated form

We report the first direct analysis of the endogenous b-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration that APC is necessary for b-catenin phosphorylation, both for priming of the prot...

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Downregulation of β-catenin by human Axin and its association with the APC tumor suppressor, β-catenin and GSK3β

Background: Inactivation of the adenomatous polyposis coli (APC) tumor suppressor protein is responsible for both inherited and sporadic forms of colon cancer. Growth control by APC may relate to its ability to downregulate β-catenin post-translationally. In cancer, mutations in APC ablate its ability to regulate β-catenin, and mutations in β-catenin prevent its downregulation by wild-type APC....

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Modeling the Effect of APC Truncation on Destruction Complex Function in Colorectal Cancer Cells

In colorectal cancer cells, APC, a tumor suppressor protein, is commonly expressed in truncated form. Truncation of APC is believed to disrupt degradation of β-catenin, which is regulated by a multiprotein complex called the destruction complex. The destruction complex comprises APC, Axin, β-catenin, serine/threonine kinases, and other proteins. The kinases CK1α and GSK -3β, which are recruited...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 2014

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.131045